Learn how a streamlined HILIC LC-MS workflow enables sensitive and reproducible analysis of underivatized amino acids in fermentation samples. Presented by Agilent Technologies at HPLC 2026, this study demonstrates amino acid profiling in spent yeast fermentation media using Agilent 1290 Infinity III Bio LC System, Altura Poroshell HILIC-Z Column, 6230C Time-of-Flight (TOF) Mass Spectrometer, MassHunter Quantitative Analysis 12.1, and MassHunter Explorer 2.0 Traditional amino acid analysis often requires derivatization, adding complexity and increasing sample preparation time. This workflow eliminates derivatization while delivering: ✅ Quantification of underivatized amino acids ✅ Separation of leucine and isoleucine isomers ✅ High mass accuracy and resolution ✅ Monitoring of nutrient uptake during fermentation ✅ Detection of organic acids and additional metabolites ✅ Untargeted metabolomics and PCA analysis Researchers can simultaneously quantify amino acids, monitor fermentation performance, and investigate metabolic changes using a metal-free HPLC flow path combined with high-resolution TOF-MS. ✅ Learn more about Agilent 1290 Infinity III Bio LC System: https://bit.ly/4hsR9VO ✅ Learn more about Agilent Altura Poroshell HILIC-Z columns: https://bit.ly/4fOyOkW ✅ Learn more about Agilent 6230C Time-of-Flight (TOF) LC/MS: https://bit.ly/3T7eB10 #HILIC #LCMS #TOFMS #MassSpectrometry #Metabolomics #Fermentation #AminoAcids #Bioprocessing #HPLC2026 #AgilentTechnologies

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